NCJ Number
222202
Journal
Forensic Science International Volume: 174 Issue: 2-3 Dated: January 2008 Pages: 182-188
Date Published
January 2008
Length
7 pages
Annotation
In order to identify the minimum number of Y-STRs (Y-chromosomal short tandem repeat loci) needed for individualizing male lineages, in addition to the recommended 9 minimal haplotype loci or the 11 SWGDAM (Scientific Working Group on DNA Analysis Methods) loci, this study assessed the haplotype discrimination capacity of the 9 Y-STR loci comprising the minimal haplotype, together with an additional 26 recently described single-copy Y-STRs, for 391 males from Germany, the Netherlands, and Turkey.
Abstract
The study showed that the typing of five Y-STRs, in addition to the nine minimal haplotype loci, revealed complete haplotype resolution and thus complete individualization of all male lineages in each of the eight population samples analyzed. In the pooled dataset of 391 males, 7 Y-STRs in addition to the 9 minimal haplotype loci revealed complete individualization. The five Y-STRs DYS447, DYS449, DYS481, DYS570, and DYS576 composed the smallest set of loci in addition to the previously recommended standard Y-STRs leading to the individualization of all males from each single population group. Complete resolution of the pooled population was achieved by the additional genotyping of two additional loci, DYS446 or DYS505 and DYF406S1 or DYS522. DNAs from unrelated persons of eight populations were used for the evaluation of the Y-STR loci. The German populations were Berlin (n=55), Sorbs (n=29), Munich (n=30), Hamburg (n=49), Dresden (n=80), and Rostock (n=36). In addition, a Dutch population of Leiden (n=72) and a Turkish population living in Berlin (n=40) were analyzed. For the amplification of the Y-STR loci, eight multiplex PCR systems were used. 5 tables and 21 references