NCJ Number
245538
Journal
Forensic Science International: Genetics Volume: 9 Dated: March 2014 Pages: 81-84
Date Published
March 2014
Length
4 pages
Annotation
A new method that enables DNA to be stored at room temperature is assessed for its ability to preserve for long periods DNA samples that mimic true mixture casework samples.
Abstract
Biomatrica Inc. has developed a sample matrix based on anhydrobiosis, which stabilizes and prevents the degradation of DNA. The samples are dried in a synthetic matrix and can be stored at room temperature. This matrix, also called Qiasafe, is a synthetic polymer that mimics the anhydrobiosis process. The protective effect is apparently based on its ability to form glass through minor groove interactions, thus stabilizing DNA. GenVault produced a similar product called GenTegra. A previous study by the authors of this report proved that medium to low quantities (150 pg/ml) of DNA can be safely stored for up to 6 months at room temperature using this method. The same study showed that multiple cycles of dehydration/hydration had no detrimental effects on the DNA. The current study complements the authors' previous work in showing that after 2 years of aging at room temperature there is a decrease in the number of observed alleles and in the peak height of these alleles. This suggests that low concentrations of mixed DNA should be stored on the matrix for shorter periods of time at room temperature (about 6 months). Also, because DNA is apparently better preserved when stored at higher concentrations, increasing the DNA concentration prior to matrix storage should be tested. Future research should also involve long-term storage experiments on the order of decades. The possibilities and limitations of methods for mimicking natural aging of DNA are discussed. 2 figures and 20 references