NCJ Number
248142
Journal
Forensic Science International Genetics Volume: 12 Dated: September 2014 Pages: 60-68
Date Published
September 2014
Length
9 pages
Annotation
The main goal of this study was to increase the discrimination power of dog mtDNA analysis by exploring SNP variation in the mtGenome coding region of the six most frequent CR haplotypes in a Belgian population sample.
Abstract
These are CR haplotypes Be47, Be23, Be19, Be01, Be03, and Be29, which respectively belong to CR haplotypes B1, A17, A11, A19, A18, and A16. Together, they represent approximately 58 percent of a Belgian population sample of 214 dogs. The entire mitochondrial genomic was sequenced for 106 of the 124 sampled dogs belonging to the six most frequent CR haplotypes, in order to estimate the frequency of the mtGenome haplotypes in the Belgian dog population. In addition, the project sequenced the mtGenome coding region of 55 dogs with other CR haplotypes,in order to assess the overall degree of variation in the mtGenome coding region. A set of 337 dog mtGenome sequences published in GenBank, of which 134 belong to the six CR haplogroups of interest, was used to assess primer specifictiy and the forensically informative nature of SNPs encountered in the authors' own study. Since whole mtGenome is not feasible for forensic trace material, the final objective of this study was to select a subset of SNPs that distinguish specimens that share one of these six CR haploptypes. 5 figures, 3 tables, and 57 references