Developmental Validation of the PowerPlex 18D System, a Rapid STR Multiplex for Analysis of Reference Samples

• NCJ Number: 241107
• Journal: Forensic Science International: Genetics Volume: 7 Issue: 1 Dated: January 2013 Pages: 129-135
• Author(s): Kathryn Oostdik; Julie French; Donald Yet; Briana Smalling; Craig Nolde; Peter M. Vallone; Erica L.R. Butts; Carolyn R. Hill; Margaret C. Kline; Theresa Rinta; Stacey R. Allen; Christopher K. Huber; John Teske; Benjamin Krenke; Martin Ensenberger; Patricia Fulmer; Cynthia Sprecher; Amy M. Gerow
• Date Published: January 2013
• Length: 7 pages
• Publication Format: Article
• Publication Type: Test/Measurement; Report (Study/Research)
• Language: English
• Country: United States of America

Annotation

In order to demonstrate suitability for human identification testing, the PowerPlex 18D System was tested for sensitivity, concordance, inhibitor tolerance, and performance with thermal cycling and reaction condition variation following SWWGDAM developmental validation guidelines.

Abstract

As short tandem repeat markers remain the foundation of human identification throughout the world, new STR multiplexes require rigorous testing to ensure the assays are sufficiently robust and reliable for genotyping purposes. The PowerPlex 18D System was created for the direct amplification of buccal and blood samples from FTA storage cards and reliably accommodates other sample materials. The PowerPlex 18D System allows simultaneous amplification of the 13 CODIS loci and amelogenin along with 4 additional loci: Penta E, Penta D, D2S1338, and D19S433. To demonstrate suitability for human identification testing, the PowerPlex 18D System was tested for sensitivity, concordance, inhibitor tolerance, and performance with thermal cycling and reaction condition variation following SWWGDAM developmental validation guidelines. Given these results, PowerPlex 18D System can confidently be used for forensic and human identification testing. (Published Abstract)