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Development of a Nomenclature System for a Canine STR Multiplex Reagent Kit

NCJ Number
231204
Journal
Journal of Forensic Sciences Volume: 55 Issue: 3 Dated: May 2010 Pages: 597-604
Author(s)
Bradley K. Tom, M.S.; Mikko T. Koskinen, Ph.D.; Melody Dayton, M.S.; Anna-Maria Mattila, M.S.; Eric Johnston, M.S.; Dennis Fantin, Ph.D.; Sue DeNise, Ph.D.; Theresa Spear, M.S.; David G. Smith, Ph.D.; Jessica Satkoski, Ph.D.; Bruce Budowle, Ph.D.; Sree Kanthaswamy, Ph.D.
Date Published
May 2010
Length
8 pages
Annotation

A nomenclature system was established based on internationally recognized recommendations for human forensic STRs for a recently developed canine STR reagent kit.

Abstract

Despite the popularity of dogs in US households, canine DNA evidence remains largely untapped in forensic investigations partially because of the absence of well-defined forensic short tandem repeats (STRs), lack of standardized and validated PCR protocols, STR reagent kits, and poorly developed nomenclature. For the current study, representative alleles were sequenced from each of the 18 STRs and the sex-typing marker included in the kit. This study also reflects on the impact of point mutations, insertions, and deletions within and outside the STR core repeat structures. An understanding of the STRs' sequence and repeat structures will enable development of a robust and reliable allele nomenclature and improve the accuracy and precision of allele fragment sizing in canine forensic profiling. The expected allele sizes have been calculated, and their repeat stuctures defined based on sequence information. 14 tables and 23 references (Published Abstract)