NCJ Number
146645
Journal
Journal of Forensic Sciences Volume: 39 Issue: 1 Dated: (January 1994) Pages: 74-85
Date Published
1994
Length
12 pages
Annotation
Methods are described for qualitatively and quantitatively determining anabolic steroids in forensic exhibits using micellar electrokinetic capillary chromatography (MECC), high performance liquid chromatography (HPLC), and capillary gas chromatography.
Abstract
Analysis of representative pharmaceutical dose forms, including a commercial aqueous suspension, a commercial tablet, several commercial oil samples, and various simulated dose units, were performed using a simple, one- step quantitative extraction procedure with methanol. Good agreement was obtained between all three techniques. A comparison of the chromatographic precision of MECC versus HPLC at the typical working concentration for anabolic steroids (approximately 0.1 mg/mL) showed that the precision for MECC migration times (less than 0.78 percent) was only slightly poorer than that observed for HPLC retention times (less than 0.53 percent). In addition, comparable peak area precisions of less than 1.9 percent were obtained for both techniques. The MECC peak height precision for methyltestosterone (1.52 percent) was significantly better than that obtained using HPLC (2.59 percent). Retention, migration, and linearity data are presented and compared for over 20 anabolic steroids commonly encountered in forensic exhibits. 12 references, 5 tables, and 8 figures