NCJ Number
246577
Journal
Journal of Forensic Sciences Volume: 59 Issue: 1 Dated: January 2014 Pages: 287-292
Date Published
January 2014
Length
6 pages
Annotation
An HPLC-DAD method was developed to detect and quantify a neonicotinoid insecticide acetamiprid ATP and its metabolite IM-1-2 in autopsy samples of a fatal intoxication case.
Abstract
An HPLC-DAD method was developed to detect and quantify a neonicotinoid insecticide acetamiprid ATP and its metabolite IM-1-2 in autopsy samples of a fatal intoxication case. The postmortem blood and tissue distribution of ATP and IM-1-2 was determined for the first time. The method showed acceptable precisions and recoveries with relative standard deviations of <10% for ATP level and 1.38 % for IM-1-2. The detection and quantification limits for ATP were 0.015 ìg/mL and 0.030 ìg/mL for blood and were 0.035 ìg/g and 0.050 ìg/g for liver samples, respectively. The mean contents of ATP were 0.79 ìg/g in the liver, 47.35 ìg/g in the stomach contents and 2.7 ìg/mL in the blood. IM-1-2 content was 17.0 ìg/g in the stomach contents. ATP and IM-1-2 were not detected in the urine. The presence of ATP and IM-1-2 in the samples was confirmed by GC-MS. The method can be exploited in future forensic casework. Abstract published by arrangement with Wiley.