Detection of Soluable ABH Blood Group Substances in Semen and Saliva Using Monoclonal Blood Grouping Reagents

• NCJ Number: 123901
• Journal: Journal of Forensic Sciences Volume: 34 Issue: 5 Dated: (September 1989) Pages: 1082-1089
• Author(s): J L Mudd
• Date Published: 1989
• Length: 8 pages
• Publication Format: Article
• Publication Type: Research (Applied/Empirical)
• Language: English
• Country: United States of America
• Note: Publication 88-05 of the Laboratory Division of the Federal Bureau of Investigation

Annotation

This study used an enzyme-linked immunosorbent assay (ELISA) to investigate the use of monoclonal antibodies for detecting secreted ABH blood group substances in semen and saliva.

Abstract

This study screened 111 semen samples and 66 saliva specimens. The results showed that the behavior of some monoclonals were unpredictable and often failed to detect the corresponding antigen in a number of the tested specimens. The suitability of the monoclonal reagents for detecting soluable blood group antigens was unpredictable by their behavior with red cell antigens. Therefore, care must be taken in the selection of monoclonal reagents for use in the detection of secreted blood group antigens as false negative results could be obtained. 7 tables, 2 notes, 16 references. (Author abstract modified)