Deoxyribonuclease I Phenotyping From Saliva Stains

• NCJ Number: 183300
• Journal: Journal of Forensic Sciences Volume: 44 Issue: 1 Dated: January 1999 Pages: 175-178
• Author(s): Reiko Iida Ph.D.; Kazumi Sawazaki Ph.D.; Toshihiro Yasuda Ph.D.; Etsuko Tsubota; Masahiro Aoyama M.D.; Takasumi Matsuki M.D.; Koichiro Kishi M.D.
• Date Published: January 1999
• Length: 4 pages
• Publication Format: Article
• Publication Type: Research (Applied/Empirical)
• Language: English
• Country: United States of America

Annotation

This paper describes a newly developed method for extraction and purification of DNase I from saliva stains.

Abstract

The research took place in Japan. DNase I phenotyping from saliva stains had previously been unsuccessful due to low enzyme activity and heavy contamination. The present research extracted salivary DNase I from stains using phosphate bufer containing Nonidet P-40. Extracts were purified using Phenyl Sepharose CL-4B gel. Electrophoresis was performed, and DNase I was successfully phenotyped. All the DNase I phenotypes obtained from saliva stains using this new method were identical to the phenotypes determined from urine samples. Moreover, this method correctly phenotyped DNase I from saliva stains that had been stored for more than 3 months at room temperature or at 37 degrees centigrade. Findings suggested that DNase I polymorphisms provide useful information for forensic characterization of saliva stains. Figures, table, and 18 references (Author abstract modified)