Autosomal SNP Typing of Forensic Samples With the GenPlex HID System: Results of a Collaborative Study

• NCJ Number: 236663
• Journal: Forensic Science International: Genetics Volume: 5 Issue: 5 Dated: November 2011 Pages: 369-375
• Author(s): C. Tomas; G. Axler-DiPerte; Z.M. Budimlija; C. Borsting; M.D. Coble; A.E. Decker; A. Eisenberg; R. Fang; M. Fondevila; S. Frisk Fredslund; S. Gonzalez; A.J. Hansen; P. Hoff-Olsen; C. Haas; P. Kohler; A.K. Kriegel; B. Lindblom; F. Manohar; O. Maronas; H.S. Mogensen; K. Neureuther; H. Nilsson; M.K. Scheible; M.L.. Sonntag; M. Stangegaard; D. Syndercombe-Court; C.R. Thacker; P.M. Vallone; A.A. Westen; N. Morling; P.M. Schneider
• Date Published: November 2011
• Length: 7 pages
• Sponsoring Agency: Ellen and Aage Andersen Foundation
• Publication Format: Article
• Publication Type: Report (Study/Research)
• Language: English
• Country: Ireland

Annotation

An international exercise was performed by 14 laboratories (9 in Europe and 5 in the US) in order to test the robustness and reliability of the GenPlex HID System on forensic samples.

Abstract

The GenPlex HID System (Applied Biosystems - AB) offers typing of 48 of the 52 SNPforID SNPs and amelogenin. Previous studies have shown a high reproducibility of the GenPlex HID System using 250-500pg DNA of good quality. In the current study, three samples with partly degraded DNA and 10 samples with low amounts of DNA were analyzed in duplicates using various amounts of DNA. In order to compare the performance of the GenPlex HID System with the most commonly used STR kits, 500pg of partly degraded DNA from three samples was typed by the laboratories using one or more STR kits. The median SNP typing success rate was 92.3 percent with 500 pg of partly degraded DNA. Three of the 14 laboratories counted for more than two-thirds of the locus dropouts. The median percentage of discrepant results was 0.2 percent with 500pg degraded DNA. An increasing percentage of locus dropouts and discrepant results were observed when lower amounts of DNA were used. Different success rates were observed for the various SNPs. The rs763869 SNP was the least successful. With the exception of the MiniFiler kit (AB), GenPlex HID performed better than five other tested STR kits. When partly degraded DNA was analyzed, GenPlex HID showed a very low mean mach probability, while all STR kits except MiniFiler had very limited discriminatory power. (Published Abstract)