NCJ Number
188637
Journal
Japanese Journal of Legal Medicine Volume: 54 Issue: 2 Dated: August 2000 Pages: 219-226
Date Published
August 2000
Length
8 pages
Annotation
This article reviews the application of the PCR-APLP method in determining ABO genotypes in forensic samples.
Abstract
ABO genotyping of forensic samples was carried out by the amplified product length polymorphism (APLP) technique. Two novel systems were presented. One was termed as the eight primers system, in which eight allele-specific primers were added into a single PCR reaction. Another was termed as the six primers system. In both APLP systems, all alleles were clearly detected using DNA purified from forensic samples. In PCR amplification with direct addition of specimen, ABO genotyping was also possible to blood stain, seminal stain, blood, saliva and urine. The PCR-APLP method was seen as convenient and valuable for forensic practice.