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APPLICATION OF THE FLUORESCENT-ANTIBODY TECHNIQUE TO BLOOD GROUP DIFFERENTIATION OF DRIED BLOODSTAINS

NCJ Number
37887
Date Published
1976
Length
246 pages
Annotation
THE FLUORESCENT ANTIBODY TECHNIQUE WAS STUDIED TO DETERMINE ITS APPLICABILITY TO BLOOD GROUP DIFFERENTIATION OF DRIED TWO-WEEK OLD BLOODSTAINS.
Abstract
INITIAL STUDIES WITH AN AMINCO-BOWMAN SPECTROPHOTOFLUOROMETER INDICATED POSSIBLE FEASIBILITY; THAT DIFFERENCES COULD BE DETECTED BETWEEN GROUP A AND B ANTIGENS REACTED WITH HOMOLOGOUS ANTIBODIES. FURTHER EXPERIMENTATION WAS DIRECTED TOWARDS DEVELOPING METHODOLOGY, REAGENTS AND INSTRUMENTATION FOR A 'LOW ANGLE, DIRECT FLUORESCENCE TAGGING METHOD'. THIS METHOD INCORPORATED THE ANALYSIS OF BLOODSTAINED FABRICS AND DRIED BLOOD PARTICULATES. A PROTOTYPE INSTRUMENT WAS DEVELOPED, AND ITS PROBLEM AREAS DEFINED. (AUTHOR ABSTRACT)