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Analysis of the NMI01 Marker for a Population Database of Cannabis Seeds

NCJ Number
Journal of Forensic Sciences Volume: 58 Issue: S 1 Dated: January 2013 Pages: S 176-S 182
Nicholas Shirley, B.S.; Lindsay Allgeier, M.S.; Tommy LaNier,; Heather Miller Coyle, Ph.D.
Date Published
January 2013
7 pages
This study analyzed the distribution of genotypes at a single hexanucleotide short tandem repeat (STR) locus in a Cannabis sativa seed database along with seed-packaging information.
This STR locus is defined by the polymerase chain reaction amplification primers CS1F and CS1R and is referred to as NMI01 (for National Marijuana Initiative) in this study. The population database consists of seed seizures of two categories: seed samples from labeled and unlabeled packages regarding seed bank source. Of a population database of 93 processed seeds including 12 labeled Cannabis varieties, the observed genotypes generated from single seeds exhibited between one and three peaks (potentially six alleles if in homozygous state). The total number of observed genotypes was 54 making this marker highly specific and highly individualizing even among seeds of common lineage. Cluster analysis associated many but not all of the handwritten labeled seed varieties tested to date as well as the National Park seizure to this known reference database containing Mr. Nice Seedbank and Sensi Seeds commercially packaged reference samples. Abstract published by arrangement with John Wiley & Sons.