NCJ Number
128380
Journal
Journal of the Association of Official Analytical Chemistry Volume: 73 Issue: 6 Dated: (1990) Pages: 902-926
Date Published
1990
Length
23 pages
Annotation
An analytical model of a reverse-phase liquid chromatographic (LC) system for screening, identifying, and quantifying the ingredients of anabolic steroid products found on the black market is presented.
Abstract
Methanolic sample extracts of these primarily oil-based injectables are chromatographed on an octedecyl column using two mobile phases and tracked at three wavelengths. A photodiode array UV detector is also used. Retention time ratios relative to testosterone and UV data are used in making tentative identifications. Identity confirmation is obtained through direct insertion probe mass spectrometry or Fourier transform infrared spectrophotometry. Recoveries for 11 different steroids extracted from sesame oil ranged from 96 to 100 percent. Values for over 40 steroids, analytical results for typical products, and spectra for selected compounds are presented. 3 tables, 13 figures, and 11 references (Author abstract modified)