This overview describes microarray-based tests that combine solution-phase amplification chemistry and microarray hybridization within a single microfluidic chamber.
The integrated biochemical approach improves microarray workflow for diagnostic applications by reducing the number of steps and minimizing the potential for sample or amplicon cross-contamination. Examples described illustrate a basic, integrated approach for DNA and RNA genomes, and a simple consumable architecture for incorporating wash steps while retaining an entirely closed system. It is anticipated that integrated microarray biochemistry will provide an opportunity to significantly reduce the complexity and cost of microarray consumables, equipment, and workflow, which in turn will enable a broader spectrum of users to exploit the intrinsic multiplexing power of microarrays for infectious disease diagnostics. (publisher abstract modified)
Downloads
Similar Publications
- Training police for procedural justice: An evaluation of officer attitudes, citizen attitudes, and police-citizen interactions
- School Resource Officers and Exclusionary Discipline in U.S. High Schools: A Systematic Review and Meta-analysis
- Machine Aided Speaker Identification - Phase One - Semiautomatic Speaker Identification System - Final Report of Phase One Research