NCJ Number
236446
Date Published
August 2007
Length
5 pages
Annotation
The purpose of this technology transfer workshop is to provide the student with an introduction to laser microdissection (LMD), methods for preparing samples for LMD, LMD operation, methods for DNA analysis of LMD recovered cells, and an understanding of the application of LMD recovery to sexual assault evidence analysis.
Abstract
The purpose of this technology transfer workshop is to provide the student with an introduction to laser microdissection (LMD), methods for preparing samples for LMD, LMD operation, methods for DNA analysis of LMD recovered cells, and an understanding of the application of LMD recovery to sexual assault evidence analysis. This class will include both lecture and laboratory activities to illustrate the concepts presented. Laser microdissection will be demonstrated on sperm and epithelial cells smears using the latest LMD instrumentation. Cells collected from the laboratory exercises will be analyzed for Short Tandem Repeat loci using PCR and capillary electrophoresis analysis.